Clathrin, Light Chain (N-Term) 抗体 (CF®647)
This anti-Clathrin, Light Chain antibody is a 小鼠 单克隆 antibody detecting Clathrin, Light Chain in IHC, WB, FACS 和 IF. Suitable for 人.
产品编号 ABIN6162999
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Clathrin, Light Chain (N-Term) 抗体 (CF®647) (ABIN6162999)
抗原
Clathrin, Light Chain
适用
人
宿主
小鼠
克隆类型
单克隆
标记
CF®647
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
CLC-1421
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抗原表位
- N-Term
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原理
- Mouse Monoclonal anti-Clathrin, Light chain (CLC/1421), CF647 Conjugate
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产品特性
- This antibody recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
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亚型
- IgG2b kappa
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anti-Clathrin, Light Chain (AA 156-173) antibody
适用: 人, 小鼠, 大鼠, 斑马鱼 IHC, WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal 57-4 unconjugated
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应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa cells. Placenta or Prostate Carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Clathrin, Light Chain
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分子量
- 31-44 kDa
抗原
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