Actin, Muscle Specific 抗体 (CF®647)
This anti-Actin, Muscle Specific antibody is a 小鼠 单克隆 antibody detecting Actin, Muscle Specific in FACS, IF, IHC (f) 和 WB. Suitable for 人, 兔 和 大鼠.
Quick Overview for Actin, Muscle Specific 抗体 (CF®647) (ABIN6162581)
抗原
Actin, Muscle Specific
适用
人, 兔, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This Actin, Muscle Specific antibody is conjugated to CF®647
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
克隆位点
MSA-953
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原理
- Mouse Monoclonal anti-Muscle-Specific-Actin (MSA/953), CF647 Conjugate
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产品特性
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Synthetic peptide common among human muscle actins
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亚型
- IgG1 kappa
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anti-Actin, Muscle Specific antibody
适用: 人, 兔, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM160 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Muscle or sarcoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Actin, Muscle Specific
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别名
- Muscle-Specific-Actin
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背景
- ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
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分子量
- Multiple
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基因ID
- 58, 59, 70
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UniProt
- P62736
抗原
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