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Desmoglein 3 抗体 (CF®640R)

This anti-Desmoglein 3 antibody is a 小鼠 单克隆 antibody detecting Desmoglein 3 in WB, IHC, IF 和 FACS. Suitable for 人.
产品编号 ABIN6162273
发货至: 中国

Quick Overview for Desmoglein 3 抗体 (CF®640R) (ABIN6162273)

抗原

See all Desmoglein 3 (DSG3) 抗体
Desmoglein 3 (DSG3)

适用

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宿主

  • 89
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小鼠

克隆类型

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单克隆

标记

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This Desmoglein 3 antibody is conjugated to CF®640R

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

5G11
  • 原理

    Mouse Monoclonal anti-Desmoglein-3 (5G11), CF640R Conjugate

    产品特性

    This antibody recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology (formalin) 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    A431 cells. Lung squamous cell carcinoma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Desmoglein 3 (DSG3)

    别名

    Desmoglein-3

    背景

    130  kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

    分子量

    130 kDa

    基因ID

    1830, 1925

    UniProt

    P32926
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