KRT1 抗体 (Biotin)
This anti-KRT1 antibody is a 小鼠 单克隆 antibody detecting KRT1 in IF, WB, FACS 和 IHC (f). Suitable for 人, 大鼠, 小鼠, 小鸡, Cow, 猴, 兔 和 犬.
Quick Overview for KRT1 抗体 (Biotin) (ABIN6161994)
抗原
See all KRT1 抗体
KRT1
(Cytokeratin HMW (KRT1))
适用
人, 大鼠, 小鼠, 小鸡, Cow, 猴, 兔, 犬
宿主
小鼠
克隆类型
单克隆
标记
This KRT1 antibody is conjugated to Biotin
应用范围
Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
克隆位点
AE-3
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原理
- Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), Biotin Conjugate
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产品特性
- This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human epidermal keratin
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亚型
- IgG1 kappa
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anti-Cytokeratin HMW (KRT1) antibody
KRT1 适用: 人, 大鼠, 小鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 34BE12 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Epithelial cells, Skin or Adenocarcinomas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- KRT1 (Cytokeratin HMW (KRT1))
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别名
- Cytokeratin, HMW
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背景
- KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
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分子量
- 52-67 kDa
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基因ID
- 51350, 654392
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UniProt
- Q01546
抗原
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