KRT1 抗体 (CF®594)
This anti-KRT1 antibody is a 小鼠 单克隆 antibody detecting KRT1 in IF, WB, FACS 和 IHC (f). Suitable for 人, 大鼠 和 小鼠.
Quick Overview for KRT1 抗体 (CF®594) (ABIN6161951)
抗原
See all KRT1 抗体
KRT1
(Cytokeratin HMW (KRT1))
适用
人, 大鼠, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
This KRT1 antibody is conjugated to CF®594
应用范围
Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
克隆位点
34BE12
-
-
原理
- Mouse Monoclonal anti-Cytokeratin, HMW (34BE12), CF594 Conjugate
-
产品特性
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Solubilized keratin extract from human stratum corneum
-
亚型
- IgG1 kappa
-
-
anti-Cytokeratin HMW (KRT1) antibody
KRT1 适用: 人, 大鼠, 小鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 34BE12 unconjugated
-
-
应用备注
-
Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
PC12 cells, Skin, Prostate carcinoma
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- KRT1 (Cytokeratin HMW (KRT1))
-
别名
- Cytokeratin, HMW
-
背景
- KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
-
分子量
- 50-67 kDa
-
基因ID
- 51350, 654392
-
UniProt
- Q01546
抗原
-
You are here:
