Cyclin B1 抗体
Quick Overview for Cyclin B1 抗体 (ABIN6161698)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Mouse Monoclonal anti-CyclinB1 (V92.1), Purified, BSA-free
-
产品特性
- This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
纯化方法
- Purified
-
免疫原
- Hamster Cyclin B1 protein
-
亚型
- IgG1 kappa
-
-
anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated
anti-Cyclin B1 (CCNB1) (C-Term) antibodyCCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 271-433) antibodyCCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-90) antibodyCCNB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-Cyclin B1 (CCNB1) (pSer126) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (Internal Region) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKO Validated CCNB1 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
-
-
应用备注
-
Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes
- Optimal dilution for a specific application should be determined by user
-
说明
-
Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS (no BSA, no azide)
-
储存液
- Azide free
-
-
- Cyclin B1 (CCNB1)
-
别名
- CyclinB1
-
背景
- CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1
-
分子量
- 55-62 kDa
-
基因ID
- 891, 23960
-
UniProt
- P14635
-
途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
-
