Keratin 10/13 抗体 (Biotin)
Quick Overview for Keratin 10/13 抗体 (Biotin) (ABIN6161006)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin 10/13 (DE-K13), Biotin Conjugate
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产品特性
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Cytoskeletal preparation extracted from human ectocervical epithelium
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亚型
- IgG2a kappa
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anti-Keratin 10/13 antibody
适用: 人, Cat, 斑马鱼 WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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应用备注
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.25-0.5 μg/mL
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说明
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A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Keratin 10/13
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别名
- Cytokeratin 10/13
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背景
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1
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分子量
- 56.5 kDa (CK10) & 53 kDa (CK13)
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基因ID
- 3858, 99936
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UniProt
- P13645
抗原
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