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CD47 抗体 (CF®594)

This anti-CD47 antibody is a 小鼠 单克隆 antibody detecting CD47 in FACS, IF 和 IHC (f). Suitable for 人 和 小鼠.
产品编号 ABIN6159747
发货至: 中国

Quick Overview for CD47 抗体 (CF®594) (ABIN6159747)

抗原

See all CD47 抗体
CD47

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD47 antibody is conjugated to CF®594

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

IAP-964 B6H12-2
  • 原理

    Mouse Monoclonal anti-CD47 (IAP/964 + B6H12.2), CF594 Conjugate

    产品特性

    This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human IAP protein (IAP/964), Intact CD47 purified from placenta (B6H12.2)

    亚型

    IgG
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    CD47

    别名

    CD47

    背景

    Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen

    分子量

    ~50 kDa

    基因ID

    961, 446414

    UniProt

    Q08722
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