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CD43 抗体 (Biotin)

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6159372
发货至: 中国

Quick Overview for CD43 抗体 (Biotin) (ABIN6159372)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

84-3C1
  • 原理

    Mouse Monoclonal anti-CD43 (84-3C1), Biotin Conjugate

    产品特性

    This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Stimulated human leukocytes

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Paracortex in a tonsil or a reactive lymph node

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    CD43

    背景

    Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

    分子量

    95, 115, or 135 kDa

    基因ID

    6693, 632188

    UniProt

    P16150

    途径

    Regulation of Leukocyte Mediated Immunity
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