CD43 抗体 (Biotin)
Quick Overview for CD43 抗体 (Biotin) (ABIN6159372)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            原理
- Mouse Monoclonal anti-CD43 (84-3C1), Biotin Conjugate
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                                            产品特性
- This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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                                            免疫原
- Stimulated human leukocytes
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                                            亚型
- IgG1 kappa
 
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                                            应用备注
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                        Immunohistology formalin-fixed 0.5-1 μg/mL- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
 
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                                            说明
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                        Paracortex in a tonsil or a reactive lymph node 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- 100 μg/mL
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                                            缓冲液
- PBS/0.1 % BSA/0.05 % azide
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
 
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    - CD43 (SPN) (Sialophorin (SPN))
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                                            别名
- CD43
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                                            背景
- Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN
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                                            分子量
- 95, 115, or 135 kDa
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                                            基因ID
- 6693, 632188
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                                            UniProt
- P16150
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                                            途径
- Regulation of Leukocyte Mediated Immunity
 抗原
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