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EpCAM 抗体 (Biotin)

This anti-EpCAM antibody is a 兔 多克隆 antibody detecting EpCAM in WB, FACS, IF 和 IHC. Suitable for 人.
产品编号 ABIN6159182
发货至: 中国

Quick Overview for EpCAM 抗体 (Biotin) (ABIN6159182)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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多克隆

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This EpCAM antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
  • 原理

    Rabbit Polyclonal anti-Ep-CAM / CD326 Biotin Conjugate

    产品特性

    EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. Ep-CAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human TACSTD1 protein

    亚型

    IgG kappa
  • 应用备注

    Immunohistology (formalin) 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HT29 cells or breast tumor

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    Ep-CAM / CD326

    分子量

    40-43 kDa

    基因ID

    4072, 542050

    UniProt

    P16422
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