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EpCAM 抗体 (N-Term) (Biotin)

This anti-EpCAM antibody is a 小鼠 单克隆 antibody detecting EpCAM in WB, FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6159068
发货至: 中国

Quick Overview for EpCAM 抗体 (N-Term) (Biotin) (ABIN6159068)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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小鼠

克隆类型

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单克隆

标记

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This EpCAM antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

EGP40-826
  • 抗原表位

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    AA 20-60, N-Term

    原理

    Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/826), Biotin Conjugate

    产品特性

    This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

    亚型

    IgG1 kappa
  • 应用备注

    Does not react with mouse or rat, others not known
    • Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HT29 cells or breast tumor

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    Ep-CAM / CD326

    背景

    Adenocarcinoma-associated Antigen| Cell Surface Glycoprotein Trop-1| EGP2| EGP314| EGP40| Epithelial Cell Adhesion Molecule| Epithelial Glycoprotein 314| ESA| KSA| TACD1| TROP1| Tumor-associated Calcium Signal Transducer 1 (TACSTD1)| ECS-1| Epidermal Surface Antigen 1| ESA1| FLOT2| Flotillin-2| Membrane Component| Chromosome 17| Surface Marker-1 (M17S1)| REG-1| Reggie-1| Reggie-2

    分子量

    40-43 kDa

    基因ID

    4072, 542050

    UniProt

    P16422
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