SELPLG 抗体 (CF®568)
Quick Overview for SELPLG 抗体 (CF®568) (ABIN6157951)
抗原
See all SELPLG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
 - Mouse Monoclonal anti-CD162 (PSGL1/1601), CF568 Conjugate
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产品特性
 - CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
 - Recombinant human full-length SELPLG (CD162) protein
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亚型
 - IgG1
 
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应用备注
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                        Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
 - Immunofluorescence 0.5-1 μg/mL
 - Flow Cytometry 0.5-1 μg/million cells/0.1 mL
 - Optimal dilution for a specific application should be determined by user
 
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说明
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Jurkat cells. Tonsil or Spleen
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 100 μg/mL
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缓冲液
 - PBS/0.1 % BSA/0.05 % azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Protect from light
 
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- SELPLG (Selectin P Ligand (SELPLG))
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别名
 - CD162
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背景
 - CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein lig, 1, PSGL1, Selectin P lig, SELPLG
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分子量
 - 43-120 kDa
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基因ID
 - 6404, 591014
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UniProt
 - Q14242
 
抗原
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