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Thrombomodulin 抗体 (AA 69-194) (CF®594)

This CF®594-conjugated 小鼠 单克隆 anti-Thrombomodulin antibody (Clone THBD-1591) specifically detects Thrombomodulin in WB, IHC, ELISA, FACS, IF, Coat 和 ISt. The antibody is reactive with 人 samples.
产品编号 ABIN6157885
发货至: 中国
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Quick Overview for Thrombomodulin 抗体 (AA 69-194) (CF®594) (ABIN6157885)

抗原

See all Thrombomodulin (THBD) 抗体
Thrombomodulin (THBD)

适用

  • 136
  • 50
  • 29
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  • 1

宿主

  • 98
  • 62
  • 8
  • 2
小鼠

克隆类型

  • 105
  • 64
  • 1
单克隆

标记

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This Thrombomodulin antibody is conjugated to CF®594

应用范围

  • 111
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunostaining (ISt)

克隆位点

THBD-1591
  • 抗原表位

    • 38
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    AA 69-194

    原理

    Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1591), CF594 Conjugate

    产品特性

    This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Immunohistology (formalin) 1-2 μg/mL
    • ELISA 2-4 μg/mL for coating (order Ab without BSA)
    •   pH 9.0
    • for 10-20 min followed by cooling at RT for 20 min
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Thrombomodulin (THBD)

    别名

    Thrombomodulin / CD141

    背景

    AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)

    分子量

    100 kDa

    基因ID

    7056, 2030

    UniProt

    P07204
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