Thrombomodulin 抗体 (AA 69-194) (CF®647)
Quick Overview for Thrombomodulin 抗体 (AA 69-194) (CF®647) (ABIN6157869)
抗原
See all Thrombomodulin (THBD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 69-194
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原理
- Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1591), CF647 Conjugate
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产品特性
- This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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应用备注
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Immunohistology (formalin) 1-2 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- pH 9.0
- for 10-20 min followed by cooling at RT for 20 min
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Thrombomodulin (THBD)
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别名
- Thrombomodulin / CD141
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背景
- AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)
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分子量
- 100 kDa
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基因ID
- 7056, 2030
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UniProt
- P07204
抗原
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