Thrombomodulin 抗体 (AA 69-194) (CF®405S)
Quick Overview for Thrombomodulin 抗体 (AA 69-194) (CF®405S) (ABIN6157861)
抗原
See all Thrombomodulin (THBD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - AA 69-194
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原理
 - Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1591), CF405S Conjugate
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产品特性
 - This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
 - Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)
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亚型
 - IgG2b kappa
 
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应用备注
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                        Immunohistology (formalin) 1-2 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
 - pH 9.0
 - for 10-20 min followed by cooling at RT for 20 min
 - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
 - Immunofluorescence 0.5-1 μg/mL
 - Flow Cytometry 0.5-1 μg/million cells/0.1 mL
 - Western blotting 0.5-1 μg/mL
 - Optimal dilution for a specific application should be determined by user
 
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说明
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THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 100 μg/mL
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缓冲液
 - PBS/0.1 % BSA/0.05 % azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Protect from light
 
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- Thrombomodulin (THBD)
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别名
 - Thrombomodulin / CD141
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背景
 - AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)
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分子量
 - 100 kDa
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基因ID
 - 7056, 2030
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UniProt
 - P07204
 
抗原
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