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VCAM1 抗体 (CF®647)

This anti-VCAM1 antibody is a 小鼠 单克隆 antibody detecting VCAM1 in FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6157679
发货至: 中国

Quick Overview for VCAM1 抗体 (CF®647) (ABIN6157679)

抗原

See all VCAM1 抗体
VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This VCAM1 antibody is conjugated to CF®647

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

1-4C3
  • 原理

    Mouse Monoclonal anti-CD106 / VCAM1 (1.4C3), CF647 Conjugate

    产品特性

    This antibody recognizes a protein of 110 kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Stimulated human umbilical vein endothelial cells (HUVEC)

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Human placenta or tonsil

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

    别名

    CD106 / VCAM1

    背景

    CD106, INCAM-100, L1CAM, Vascular Cell Adhesion Molecule 1 (VCAM1), Vascular cell adhesion protein 1

    分子量

    ~110 kDa

    基因ID

    7412, 109225

    UniProt

    P19320

    途径

    Carbohydrate Homeostasis
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