Melanoma Marker 抗体 (CF®594)
This anti-Melanoma Marker antibody is a 小鼠 单克隆 antibody detecting Melanoma Marker in IHC (f) 和 ISt. Suitable for 人.
Quick Overview for Melanoma Marker 抗体 (CF®594) (ABIN6157087)
抗原
Melanoma Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Melanoma Marker antibody is conjugated to CF®594
应用范围
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
克隆位点
HMB45 A103 T311
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原理
- Mouse Monoclonal anti-Melanoma Marker (HMB45 + A103 + T311), CF594 Conjugate
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产品特性
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant hMART-1 protein (A103), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
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亚型
- IgG1, IgG2a kappa
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anti-Melanoma Marker antibody
适用: 人 IHC, IF 宿主: 小鼠 Monoclonal KBA-62 unconjugated
anti-Melanoma Marker antibody适用: 人, 犬, 小鼠 IHC, IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
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应用备注
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Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Optimal dilution for a specific application should be determined by user
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说明
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SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Melanoma Marker
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背景
- Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17
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分子量
- 20-22 kDa (doublet) (MART), 70-80 kDa (Tyrosinase), 90-100 kDa (gp100)
抗原
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