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VWF 抗体 (AA 1815-1939) (CF®488A)

This anti-VWF antibody is a 小鼠 单克隆 antibody detecting VWF in WB, IHC, IP, FACS 和 IF. Suitable for 人.
产品编号 ABIN6154615
发货至: 中国

Quick Overview for VWF 抗体 (AA 1815-1939) (CF®488A) (ABIN6154615)

抗原

See all VWF 抗体
VWF (Von Willebrand Factor (VWF))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This VWF antibody is conjugated to CF®488A

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

VWF-1465
  • 抗原表位

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    AA 1815-1939

    原理

    Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1465), CF488A Conjugate

    产品特性

    von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

    亚型

    IgG1
  • 应用备注

    Immunohistology (formalin) 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HUVEC cells or Tonsil

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    VWF (Von Willebrand Factor (VWF))

    别名

    von-Willebrand Factor / Factor-VIII-Related Ag

    背景

    Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)

    分子量

    250 kDa

    基因ID

    7450, 440848

    UniProt

    P04275
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