TOP1MT 抗体 (CF®647)
Quick Overview for TOP1MT 抗体 (CF®647) (ABIN6154259)
抗原
See all TOP1MT 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF647 Conjugate
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产品特性
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human TOP1MT protein
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亚型
- IgG2b kappa
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anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibody
TOP1MT 适用: 人 IHC, WB, IF, FACS, StM 宿主: 小鼠 Monoclonal TOP1MT-488 unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TOP1MT-568 unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 470-550) antibodyTOP1MT 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 IHC, StM 宿主: 兔 Monoclonal TOP1MT-2883R unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 270-400) antibodyTOP1MT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (Internal Region) antibodyTOP1MT 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 11-238) antibodyTOP1MT 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 1-238) antibodyTOP1MT 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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A431 cells. Heart, Skeletal muscle, brain or fetal liver.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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别名
- Topoisomerase-I, MT
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背景
- DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase
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分子量
- 70 kDa
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基因ID
- 116447, 528574
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UniProt
- Q969P6
抗原
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