HLA-DP beta 抗体
This anti-HLA-DP beta antibody is a 小鼠 单克隆 antibody detecting HLA-DP beta in FACS, IF 和 IHC (f). Suitable for 人.
Quick Overview for HLA-DP beta 抗体 (ABIN6153336)
抗原
HLA-DP beta
(MHC Class II HLA-DP beta (HLA-DP beta))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This HLA-DP beta antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
克隆位点
BRA-FB6
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原理
- Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), Purified, with BSA
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产品特性
- This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
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亚型
- IgG2b kappa
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anti-MHC Class II HLA-DP beta (HLA-DP beta) antibody
HLA-DP beta 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal Bra-14 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Raji cells. Tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
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别名
- MHC-II (HLA-DP) (beta chain)
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分子量
- 33-35 kDa
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基因ID
- 3115, 347270
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UniProt
- P04440
抗原
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