Blood Group Lewis Y 抗体 (CF®647)
适用: 人
FACS, IF, IHC
宿主: 小鼠
Monoclonal
A70-A-A9
CF®647
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- 抗原
- Blood Group Lewis Y
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- CF®647
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 原理
- Mouse Monoclonal anti-Lewis-Y (A70-A/A9), CF647 Conjugate
- 产品特性
- This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Live Ls174T cells (human colon carcinoma cell line)
- 克隆位点
- A70-A-A9
- 亚型
- IgG1 kappa
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anti-Blood Group Lewis Y antibody
适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal A70-A-A9 unconjugated
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- 应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Lung, Colon, Bladder or Hepatocellular Carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Blood Group Lewis Y
- 别名
- Lewis-Y
- 背景
- Lewis Y antigen
- 分子量
- Multiple
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