CRYAB 抗体 (C-Term)
Quick Overview for CRYAB 抗体 (C-Term) (ABIN615274)
抗原
See all CRYAB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody reacts to Alpha Crystallin B Chain (CRYAB).
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Porcine, Bovine and Rabbit.
Species reactivity (tested):Human. -
纯化方法
- Protein A Chromatography
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免疫原
- CRYAB antibody was raised against synthetic peptide derived from sequence near the carboxy-terminus of human alphaB-Crystallin conjugated to KLH .Remarks: Sequence is identical in rat, rabbit, and bovine.
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anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibody
CRYAB 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 6G5 unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-CRYAB-1 unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1D11C6E6 unconjugated
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Crystallin, alpha B (CRYAB) antibody (FITC)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 小鼠 Monoclonal 3A10-C9 HRP
anti-Crystallin, alpha B (CRYAB) antibody (Biotin)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- PBS containing 0.09 % sodium azide as preservative and 50 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CRYAB (Crystallin, alpha B (CRYAB))
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别名
- alpha-Crystallin B Chain
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背景
- Alpha-crystallins composed of ~20 kDa alphaA and alphaB subunits function as major water-soluble proteins accounting for almost 50 % of total protein in the mammalian transparent eye lens, also existing in a variety of other tissues1. Crystallin families beta and gamma share homology with each other but not the alpha-crystallin family or the small heat shock protein (sHsp) family. sHsps including the alpha- crystallin proteins are induced by heat and other stress insults in a variety of organisms. The alpha-crystallins possess structural and functional similarities and share sequence homology with Hsp25/273. Most sHsps exhibit four common structural and functional features: monomeric molecular weight between 12 and 43 kDa, the formation of large oligomeric complexes especially for alphaA-crystallin, alphaB-crystallin and Hsp25/27, a moderately conserved alpha-crystallin domain in the central region of the protein, and molecular chaperone activity. The alpha-crystallin domain bounded by variable N-terminal and C-terminal extensions contains approximately 80 residues and participates in oligomer assembly. Oligomers, potentially 800 kDa or more, exhibit dynamic subunit exchanges and organizational plasticity, which may promote functional diversity. Phosphorylation of serine residues specifically for Hsp27 occurs in response to stress during development, typically decreasing oligomer size. Chaperone activity requires oligomerization (which, in turn, modulates the chaperone activity) and is confined to binding unfolded intermediates to prevent irreversible aggregation, even though productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.Synonyms: Alpha(B)-crystallin, CRYA2, CRYAB, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
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基因ID
- 1410
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NCBI登录号
- NP_001876
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UniProt
- P02511
抗原
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