EAF2 抗体 (AA 1-170)
Quick Overview for EAF2 抗体 (AA 1-170) (ABIN6139905)
抗原
See all EAF2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-170
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序列
- MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM
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交叉反应
- 人, 小鼠, 大鼠
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产品特性
- Polyclonal Antibodies
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纯化方法
- Affinity purification
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免疫原
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).
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亚型
- IgG
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anti-ELL Associated Factor 2 (EAF2) (AA 1-170) antibody
EAF2 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-ELL Associated Factor 2 (EAF2) (AA 6-100) antibodyEAF2 适用: 人 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-ELL Associated Factor 2 (EAF2) (AA 1-260) antibodyEAF2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- WB,1:500 - 1:2000,IF,1:50 - 1:100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- EAF2 (ELL Associated Factor 2 (EAF2))
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别名
- EAF2
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背景
- Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF2,BM040,TRAITS,U19,Epigenetics & Nuclear Signaling,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Apoptosis,EAF2
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分子量
- 14 kDa/28 kDa
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基因ID
- 55840
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UniProt
- Q96CJ1
抗原
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