Vinculin 抗体
Quick Overview for Vinculin 抗体 (ABIN604849)
抗原
See all Vinculin (VCL) 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes human vinculin.
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纯化方法
- Protein G purified
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免疫原
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Semi-purified vinculin. MW of Antigen: 130kD (vinculin) and 150kD (meta-vinculin). Cellular Localization: Cell membrane and cytoplasmic.
Type of Immunogen: Protein extract -
亚型
- IgG1
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应用备注
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Approved: IF, IHC, IHC-P (2.5 - 5 μg/mL), IP, WB (1 - 2 μg/mL)
Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Immunoprecipitation (Denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2hrs at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4. No preservatives or stabilizing proteins added.
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储存液
- Azide free
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Vinculin (VCL)
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别名
- VCL / Vinculin
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背景
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Name/Gene ID: VCL
Family: Cytoskeleton
Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL -
基因ID
- 7414
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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