IDUA 抗体 (AA 26-653)
Quick Overview for IDUA 抗体 (AA 26-653) (ABIN603316)
抗原
See all IDUA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 26-653
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特异性
- Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79 % ), chicken (61 %).
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纯化方法
- Protein G purified
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免疫原
- NS0-derived, recombinant human IDUA aa26-653.
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亚型
- IgG1
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
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注意事项
- Aliquot to Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." in: Biotechnology and bioengineering, (2022) (PubMed).
: "Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase." in: Plant biotechnology journal, Vol. 17, Issue 2, pp. 505-516, (2019) (PubMed).
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Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." in: Biotechnology and bioengineering, (2022) (PubMed).
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- IDUA (Iduronidase, alpha-L- (IDUA))
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别名
- IDUA / MPS1
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背景
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Name/Gene ID: IDUA
Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA -
基因ID
- 3425
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途径
- Glycosaminoglycan Metabolic Process
抗原
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