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IDUA 抗体 (AA 26-653)

Cited in 2+ publications. This 小鼠 单克隆 anti-IDUA antibody (Clone 452619) specifically detects IDUA in ELISA 和 WB. The antibody is reactive with 人 samples.
产品编号 ABIN603316
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IDUA 抗体 (AA 26-653) (ABIN603316)

抗原

See all IDUA 抗体
IDUA (Iduronidase, alpha-L- (IDUA))

适用

  • 36
  • 15
  • 5
  • 4
  • 4
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  • 1
  • 1

宿主

  • 34
  • 2
  • 1
小鼠

克隆类型

  • 36
  • 1
单克隆

标记

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  • 4
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  • 2
  • 1
  • 1
  • 1
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This IDUA antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB)

克隆位点

452619
  • 抗原表位

    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    AA 26-653

    特异性

    Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79 % ), chicken (61 %).

    纯化方法

    Protein G purified

    免疫原

    NS0-derived, recombinant human IDUA aa26-653.

    亚型

    IgG1
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.4, 5 % trehalose

    注意事项

    Aliquot to Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
  • Gutierrez-Valdes, Häkkinen, Lemasson, de Groot, Ele-Ekouna, Guillet, Cardon, Ritala: "Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." in: Biotechnology and bioengineering, (2022) (PubMed).

    Cardon, Pallisse, Bardor, Caron, Vanier, Ele Ekouna, Lerouge, Boitel-Conti, Guillet: "Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase." in: Plant biotechnology journal, Vol. 17, Issue 2, pp. 505-516, (2019) (PubMed).

  • 抗原

    IDUA (Iduronidase, alpha-L- (IDUA))

    别名

    IDUA / MPS1

    背景

    Name/Gene ID: IDUA

    Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA

    基因ID

    3425

    途径

    Glycosaminoglycan Metabolic Process
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