LGALS14 抗体 (AA 1-139)
Quick Overview for LGALS14 抗体 (AA 1-139) (ABIN603241)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-139
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特异性
- Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.
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纯化方法
- Immunoaffinity purified
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过滤
- Sterile filtered
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免疫原
- Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14, aa 1-139, Accession# Q8TCE9).
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亚型
- IgG2b
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)
Usage: Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10 μg total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris, pH 7.4 5 % nonfat dry milk 5 % nonfat dry milk 0.15 M NaCl in Blotting Buffer in Blotting Buffer 0.05 % Tween 20. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from sterile-filtered PBS, 5 % trehalose
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
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- LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))
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别名
- LGALS14 / CLC2
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背景
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Name/Gene ID: LGALS14
Synonyms: LGALS14, CLC2, Gal-14, Galectin-14, Placental protein 13-like, PPL13 -
基因ID
- 56891
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UniProt
- Q8TCE9
抗原
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