MYOD1 抗体 (AA 77-215)
Quick Overview for MYOD1 抗体 (AA 77-215) (ABIN601982)
抗原
See all MYOD1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 77-215
-
特异性
- Recognizes human MyoD.
-
纯化方法
- Protein G purified
-
过滤
- 0.2 μm filtered
-
免疫原
-
Recombinant human MyoD, aa 77-215 (E. coli). Accession# (P15172).
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
-
-
-
-
应用备注
-
Approved: IHC (10 μg/mL)
Usage: Immunohistochemistry:10 μg/mL to detect MyoD in C2C12 mouse muscle myoblast cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with. PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for. 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4 °C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Sterile 40-50 % glycerol, PBS.
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose
-
储存条件
- -20 °C
-
储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
-
-
- MYOD1 (Myogenic Differentiation 1 (MYOD1))
-
别名
- MYOD / MYOD1
-
背景
-
Name/Gene ID: MYOD1
Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3 -
基因ID
- 4654
-
UniProt
- P15172
-
途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
-