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MYOD1 抗体 (AA 77-215)

The 小鼠 单克隆 anti-MYOD1 antibody is suitable to detect MYOD1 in samples from 人. It has been validated for IHC.
产品编号 ABIN601982
发货至: 中国
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Quick Overview for MYOD1 抗体 (AA 77-215) (ABIN601982)

抗原

See all MYOD1 抗体
MYOD1 (Myogenic Differentiation 1 (MYOD1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MYOD1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)
  • 抗原表位

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    AA 77-215

    特异性

    Recognizes human MyoD.

    纯化方法

    Protein G purified

    过滤

    0.2 μm filtered

    免疫原

    Recombinant human MyoD, aa 77-215 (E. coli). Accession# (P15172).

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a
  • 应用备注

    Approved: IHC (10 μg/mL)

    Usage: Immunohistochemistry:10 μg/mL to detect MyoD in C2C12 mouse muscle myoblast cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with. PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for. 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4 °C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile 40-50 % glycerol, PBS.

    浓度

    Lot specific

    缓冲液

    Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose

    储存条件

    -20 °C

    储存方法

    Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
  • 抗原

    MYOD1 (Myogenic Differentiation 1 (MYOD1))

    别名

    MYOD / MYOD1

    背景

    Name/Gene ID: MYOD1

    Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3

    基因ID

    4654

    UniProt

    P15172

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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