PRSS3 抗体
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北京 101111
Quick Overview for PRSS3 抗体 (ABIN598236)
抗原
See all PRSS3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96 % , rat: 76 %, mouse: 74 %, dog: 72 %. and chicken: 67 %. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.
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纯化方法
- Protein G purified
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免疫原
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NS0-derived recombinant human rhTrypsin-3.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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anti-Protease, serine, 3 (PRSS3) (AA 81-303) antibody
PRSS3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 136-163) antibodyPRSS3 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31598 unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 13-40), (N-Term) antibodyPRSS3 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24403 unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 143-171), (Middle Region) antibodyPRSS3 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 20-48), (N-Term) antibodyPRSS3 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 13-40), (N-Term) antibodyPRSS3 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) (N-Term) antibodyPRSS3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) (AA 71-120) antibodyPRSS3 适用: 人, 大鼠, 小鸡, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protease, serine, 3 (PRSS3) antibody (HRP)PRSS3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-Protease, serine, 3 (PRSS3) antibody (Biotin)PRSS3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
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应用备注
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Approved: Neut, WB (1 - 2 μg/mL)
Usage: Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 0.15 μg/mL (IC 50) under conditions in which the enzyme was present at 0.03 μg/mL and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme. Western blot:1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile buffer.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- PRSS3 (Protease, serine, 3 (PRSS3))
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别名
- PRSS3 / Trypsin 3
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背景
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Name/Gene ID: PRSS3
Subfamily: Serine S1
Family: Protease
Synonyms: PRSS3, Brain trypsinogen, Mesotrypsin, PRSS4, Serine protease 4, Serine protease 3, TRY4, Trypsin III, Trypsin IV, Trypsinogen 4, MTG, Trypsinogen 5, Pancreatic trypsinogen III, Protease, serine, 3, Trypsin-3, Mesotrypsinogen, T9, TRY3, Trypsinogen IV -
基因ID
- 5646
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UniProt
- P35030
抗原
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