NOX5 抗体 (Internal Region)
Quick Overview for NOX5 抗体 (Internal Region) (ABIN571285)
抗原
See all NOX5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- NOX5
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序列
- EWHPFTISSA PEQKD
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特异性
- This antibody is expected to recognize all reported isoforms (NP_078781.3, NP_001171708.1, NP_001171709.1).
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交叉反应
- Cow, 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- C-EWHPFTISSAPEQKD
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亚型
- IgG
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应用备注
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Western Blot: Approx 90 kDa band observed in Human Testis lysates (calculated MW of 86.4 kDa according to NP_078781.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- NOX5 (NADPH Oxidase 5 (NOX5))
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别名
- NOX5
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背景
- NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444
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基因ID
- 79400
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NCBI登录号
- NP_078781, NP_001171708, NP_001171709
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途径
- Proton Transport
抗原
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