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- 抗原
- HLA-A+B+C+E
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- 原理
- Anti-HLA-ABCE Purified
- 特异性
- The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- IFN-gamma-treated human melanoma cells Colo 38
- 克隆位点
- TP25-99SF
- 亚型
- IgG1
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anti-HLA-A+B+C+E antibody (PE)
适用: 人 FACS 宿主: 小鼠 Monoclonal TP25-99SF PE
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- 应用备注
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Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions. - 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- HLA-A+B+C+E
- 别名
- HLA-ABCE
- 背景
- HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
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