HLA-A+B+C+E 抗体
This 小鼠 单克隆 antibody specifically detects HLA-A+B+C+E in FACS, IHC (fro) 和 WB. It exhibits reactivity toward 人.
产品编号 ABIN5711913
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for HLA-A+B+C+E 抗体 (ABIN5711913)
抗原
HLA-A+B+C+E
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
克隆位点
TP25-99SF
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原理
- Anti-HLA-ABCE Purified
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特异性
- The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- IFN-gamma-treated human melanoma cells Colo 38
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亚型
- IgG1
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anti-HLA-A+B+C+E antibody (PE)
适用: 人 FACS 宿主: 小鼠 Monoclonal TP25-99SF PE
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应用备注
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Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- HLA-A+B+C+E
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别名
- HLA-ABCE
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背景
- HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
抗原
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