Dystroglycan 抗体 (Internal Region)
Quick Overview for Dystroglycan 抗体 (Internal Region) (ABIN571179)
抗原
See all Dystroglycan (DAG1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- DAG1
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序列
- HVGKHEYFMH ATDK
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特异性
- This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- C-HVGKHEYFMHATDK
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
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别名
- DAG1
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背景
- DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1
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基因ID
- 1605
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NCBI登录号
- NP_004384
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途径
- Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
抗原
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