RDH5 抗体 (AA 19-31)
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Quick Overview for RDH5 抗体 (AA 19-31) (ABIN571175)
抗原
See all RDH5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 19-31
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原理
- RDH5 (aa 19-31)
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序列
- RDRQSLPASN AFV
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RDRQSLPASNAFV, from the internal region of the protein sequence according to NP_002896.2.
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亚型
- IgG
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anti-Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5) antibody
RDH5 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5) (AA 24-318) antibodyRDH5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5) (AA 181-280) antibodyRDH5 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration: 5-10 μg/mL.
Western Blot: Preliminary testing showed a band at approx..32 kDa in Human Heart and approx. 35 kDa in Human Breast lysate, after 0.3 μg/mL antibody staining. (calculated MW of 35.0 kDa according to NP_002896.2). Primary incubation 1 hour at room temperatur
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- RDH5 (Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5))
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别名
- RDH5
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背景
- RDH5, retinol dehydrogenase 5 (11-cis/9-cis), FLJ39337, FLJ97089, HSD17B9, RDH1, SDR9C5, 11-cis RDH, 9-cis-retinol specific dehydrogenase, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), short chain dehydrogenase/reductase family 9C,
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基因ID
- 5959, 19682
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NCBI登录号
- NP_002896
抗原
-
