POU3F3 抗体 (Internal Region)
Quick Overview for POU3F3 抗体 (Internal Region) (ABIN571161)
抗原
See all POU3F3 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- POU3F3 / BRN1 / OCT8
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序列
- HMLSHAHQWV TAL
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri
Peptide ELISA: antibody detection limit dilution 1:4000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
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别名
- POU3F3
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背景
- POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8
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基因ID
- 5455, 18993, 192109
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NCBI登录号
- NP_006227
抗原
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