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POU3F3 抗体 (Internal Region)

This anti-POU3F3 antibody is a 山羊 多克隆 antibody detecting POU3F3 in WB, ELISA, FACS 和 IF. Suitable for 小鼠.
产品编号 ABIN571161
发货至: 中国

Quick Overview for POU3F3 抗体 (Internal Region) (ABIN571161)

抗原

See all POU3F3 抗体
POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

适用

  • 15
  • 3
  • 3
  • 3
  • 1
  • 1
小鼠

宿主

  • 16
  • 1
山羊

克隆类型

  • 17
多克隆

标记

  • 12
  • 1
  • 1
  • 1
  • 1
  • 1
This POU3F3 antibody is un-conjugated

应用范围

  • 17
  • 12
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    POU3F3 / BRN1 / OCT8

    序列

    HMLSHAHQWV TAL

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

    别名

    POU3F3

    背景

    POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8

    基因ID

    5455, 18993, 192109

    NCBI登录号

    NP_006227
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