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AIM2 抗体 (C-Term)

This anti-AIM2 antibody is a 山羊 多克隆 antibody detecting AIM2 in WB, ELISA, IHC, IF 和 FACS. Suitable for 人.
产品编号 ABIN571131
发货至: 中国

Quick Overview for AIM2 抗体 (C-Term) (ABIN571131)

抗原

See all AIM2 抗体
AIM2 (Absent in Melanoma 2 (AIM2))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This AIM2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    AIM2

    序列

    GEKLQLTSGV HSTIK

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.

    Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    AIM2 (Absent in Melanoma 2 (AIM2))

    别名

    AIM2

    背景

    AIM2, absent in melanoma 2, PYHIN4

    分子量

    39.0kDa

    基因ID

    9447

    NCBI登录号

    NP_004824

    途径

    Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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