Tppp 抗体 (Internal Region)
Quick Overview for Tppp 抗体 (Internal Region) (ABIN571099)
抗原
See all Tppp 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- TPPP/p25
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序列
- DPSKDRAAKR LSLES
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- C-DPSKDRAAKRLSLES
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
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别名
- TPPP
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背景
- TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
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基因ID
- 11076, 72948, 361466
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NCBI登录号
- NP_008961
抗原
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