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Sorting Nexin 1 抗体 (Internal Region)

This 山羊 多克隆 anti-Sorting Nexin 1 antibody specifically detects Sorting Nexin 1 in WB, ELISA, IHC 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN571095
发货至: 中国
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Quick Overview for Sorting Nexin 1 抗体 (Internal Region) (ABIN571095)

抗原

See all Sorting Nexin 1 (SNX1) 抗体
Sorting Nexin 1 (SNX1)

适用

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宿主

  • 71
  • 4
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山羊

克隆类型

  • 56
  • 22
多克隆

标记

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This Sorting Nexin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

质量等级

Verified
  • 抗原表位

    • 15
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    Internal Region

    原理

    SNX1

    序列

    TNSSKPQPTY EELE

    特异性

    This antibody is expected to recognize isoform a and c (NP_003090.2, NP_690039.1.

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2, NP_690039.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5 μg/mL.

    Western Blot: Approx 75 kDa band observed in lysates of cell lines HeLa and U2OS (calculated MW of 59.1 kDa according to NP_003090.2). This molecular weight is routinely observed by other sources . Recommended concentration: 0.1-1 μg/mL. Primary incubation

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Sorting Nexin 1 (SNX1)

    别名

    SNX1

    背景

    SNX1, sorting nexin 1, HsT17379, MGC8664, SNX1A, Vps5, sorting nexin 1A

    基因ID

    6642, 56440, 84471

    NCBI登录号

    NP_003090, NP_690039
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