Sorting Nexin 1 抗体 (Internal Region)
Quick Overview for Sorting Nexin 1 抗体 (Internal Region) (ABIN571095)
抗原
See all Sorting Nexin 1 (SNX1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- SNX1
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序列
- TNSSKPQPTY EELE
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特异性
- This antibody is expected to recognize isoform a and c (NP_003090.2, NP_690039.1.
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2, NP_690039.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HeLa and U2OS (calculated MW of 59.1 kDa according to NP_003090.2). This molecular weight is routinely observed by other sources . Recommended concentration: 0.1-1 μg/mL. Primary incubation
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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