ITGAL 抗体 (Internal Region)
Quick Overview for ITGAL 抗体 (Internal Region) (ABIN571017)
抗原
See all ITGAL 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- CD11A / integrin alpha L
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序列
- YQVRIQPSIH DHN
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特异性
- This antibody is expected to recognize both reported isoforms (NP_002200.2, NP_001107852.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2, NP_001107852.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 200 kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129 kDa according to NP_002200.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ITGAL (Integrin alpha-L (ITGAL))
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别名
- ITGAL
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背景
- ITGAL, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), CD11A, LFA-1, LFA1A, LFA-1 alpha, antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide, integrin alpha L, integrin g
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分子量
- 129kDa
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基因ID
- 3683
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NCBI登录号
- NP_002200, NP_001107852
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途径
- Activated T Cell Proliferation, Integrin Complex
抗原
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