ITM2B 抗体 (Internal Region)
Quick Overview for ITM2B 抗体 (Internal Region) (ABIN570955)
抗原
See all ITM2B 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- ITM2B
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序列
- HDKETYKLQR RETIK
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交叉反应
- Cow, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
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亚型
- IgG
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anti-Integral Membrane Protein 2B (ITM2B) (AA 81-148) antibody
ITM2B 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 1-266) antibodyITM2B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 1-266) antibodyITM2B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 146-245) antibodyITM2B 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 218-247), (C-Term) antibodyITM2B 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB32712 unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ITM2B (Integral Membrane Protein 2B (ITM2B))
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别名
- ITM2B
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背景
- ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394
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基因ID
- 9445, 16432, 290364
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NCBI登录号
- NP_068839
抗原
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