IKZF4 抗体 (Internal Region)
Quick Overview for IKZF4 抗体 (Internal Region) (ABIN570827)
抗原
See all IKZF4 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- IKZF4
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序列
- RPTFIDRLAN SLTKR
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特异性
- This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1
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交叉反应
- Cow, 犬, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.
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亚型
- IgG
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应用备注
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Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0
Peptide ELISA: antibody detection limit dilution 1:2000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))
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别名
- IKZF4
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背景
- IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos
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基因ID
- 64375, 22781
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NCBI登录号
- NP_071910
抗原
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