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IKZF4 抗体 (Internal Region)

The 山羊 多克隆 anti-IKZF4 antibody is suitable to detect IKZF4 in samples from 人 和 小鼠. It has been validated for WB, ELISA, IF 和 FACS.
产品编号 ABIN570827
发货至: 中国
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Quick Overview for IKZF4 抗体 (Internal Region) (ABIN570827)

抗原

See all IKZF4 抗体
IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

适用

  • 26
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
人, 小鼠

宿主

  • 21
  • 5
山羊

克隆类型

  • 24
  • 2
多克隆

标记

  • 19
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This IKZF4 antibody is un-conjugated

应用范围

  • 17
  • 8
  • 4
  • 4
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    IKZF4

    序列

    RPTFIDRLAN SLTKR

    特异性

    This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1

    交叉反应

    Cow, 犬, 人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0

    Peptide ELISA: antibody detection limit dilution 1:2000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

    别名

    IKZF4

    背景

    IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos

    基因ID

    64375, 22781

    NCBI登录号

    NP_071910
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