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KRT8 抗体

This anti-KRT8 antibody is a 兔 单克隆 antibody detecting KRT8 in IHC (p). Suitable for 人.
产品编号 ABIN5707853
发货至: 中国

Quick Overview for KRT8 抗体 (ABIN5707853)

抗原

See all KRT8 抗体
KRT8 (Keratin 8 (KRT8))

适用

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宿主

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克隆类型

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单克隆

标记

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This KRT8 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

KRT8-2174R
  • 纯化方法

    Purified

    纯度

    Protein A affinity chromatography

    免疫原

    A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant Cytokeratin 8 antibody.

    亚型

    IgG kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Cytokeratin 8 antibody to be titered up or down for optimal performance.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant Cytokeratin 8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    KRT8 (Keratin 8 (KRT8))

    别名

    KRT8 / Cytokeratin 8

    背景

    Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
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