Nebulette 抗体 (Internal Region)
Quick Overview for Nebulette 抗体 (Internal Region) (ABIN570759)
抗原
See all Nebulette (NEBL) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- NEBL / nebulette
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序列
- ELQRLKRTQE
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特异性
- This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1, NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
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交叉反应
- 犬, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1, NP_001166955.1.
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亚型
- IgG
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anti-Nebulette (NEBL) antibody
NEBL 适用: 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nebulette (NEBL) (AA 360-403) antibodyNEBL 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nebulette (NEBL) (AA 438-533) antibodyNEBL 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Preliminary experiments gave an approx 30 kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/mL antibody staining. This protein has a calculated MW of 31.2 kDa according to NP_998734.1. T
Peptide ELISA: antibody detection limit dilution 1:4000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nebulette (NEBL)
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别名
- NEBL
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背景
- NEBL, nebulette, FLJ53769, LNEBL, MGC119746, MGC119747, bA56H7.1, LIM-nebulette, NEBL, actin-binding Z-disc protein, OTTHUMP00000019268, actin-binding Z-disk protein
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基因ID
- 10529, 74103
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NCBI登录号
- NP_998734, NP_001166955
抗原
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