CD43 抗体
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北京 101111
Quick Overview for CD43 抗体 (ABIN5707565)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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纯度
- Protein A affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.
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亚型
- IgG kappa
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应用备注
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Optimal dilution of the recombinant CD43 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- CD43
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背景
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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