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Recombinant EpCAM 抗体 (Extracellular)

The 小鼠 单克隆 anti-EpCAM antibody (Clone rEGP40-1372) (ABIN5707561) specifically detects EpCAM in WB 和 IHC (p). The antibody is reactive with 人, Cat 和 犬 samples.
产品编号 ABIN5707561
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant EpCAM 抗体 (Extracellular) (ABIN5707561)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

抗体类型

Recombinant Antibody

适用

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人, Cat, 犬

宿主

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  • 224
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  • 1
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小鼠

克隆类型

  • 396
  • 121
  • 3
单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

  • 305
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

REGP40-1372
  • 抗原表位

    • 86
    • 52
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    • 35
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    AA 100-224, Extracellular

    原理

    Recombinant EpCAM Antibody / Extracellular domain (azide and preservative free)

    纯化方法

    Protein G affinity chromatography

    免疫原

    A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.

    亚型

    IgG1, kappa
  • 应用备注

    Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    EpCAM

    背景

    EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    UniProt

    P16422
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