Thrombomodulin 抗体 (AA 69-194)
Quick Overview for Thrombomodulin 抗体 (AA 69-194) (ABIN5707539)
抗原
See all Thrombomodulin (THBD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 69-194
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this recombinant Thrombomodulin antibody.
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亚型
- IgG1 kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Thrombomodulin antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Thrombomodulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Thrombomodulin (THBD)
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别名
- Thrombomodulin / CD141
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背景
- It recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.
抗原
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