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SHMT1 抗体

This anti-SHMT1 antibody is a 兔 多克隆 antibody detecting SHMT1 in WB. Suitable for Vicia faba, Spinach, Pisum sativum 和 Arabidopsis thaliana. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN5706931
发货至: 中国

Quick Overview for SHMT1 抗体 (ABIN5706931)

抗原

See all SHMT1 抗体
SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

适用

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Vicia faba, Spinach, Pisum sativum, Arabidopsis thaliana

宿主

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克隆类型

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多克隆

标记

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This SHMT1 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 特异性

    The antibody is very specific for salmonid inducible form - hsp70.

    交叉反应 (详细)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    预测反应

    dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii

    产品特性

    Expected / apparent Molecular Weight of the Antigene: 53 kDa (Arabidopsis thaliana)

    纯化方法

    affinity purified

    免疫原

    Purified SHMT protein from Spinacia oleracea

    亚型

    IgG
  • 应用备注

    1:5 000 with standard ECL (WB)

    说明

    this antibody can be used on total leaf extracts and isolated mitochondria

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 200 μL of sterile water.

    缓冲液

    PBS pH 7.4

    注意事项

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Wei, Sun, Sandoval, Cross, Gordon, Kang, Roje: "Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana." in: Archives of biochemistry and biophysics, Vol. 536, Issue 1, pp. 87-96, (2013) (PubMed).

  • 抗原

    SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

    别名

    SHMT

    背景

    Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

    分子量

    53 kDa (Arabidopsis thaliana)
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