(1 reference)-
- 抗原 See all Luciferase 抗体
- Luciferase
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适用
- Vibrio fischeri
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Luciferase antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 产品特性
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Synonyms: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB
Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.
Gene Name: LUXB_VIBFI, luxA
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Photobacterium fischerii]. Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.
- 免疫原
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Immunogen: Luciferase [Photobacterium fischerii]
Immunogen Type: Native Protein
- Top Product
- Discover our top product Luciferase Primary Antibody
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anti-Luciferase antibody
适用: Firefly WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 6B8 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly ELISA, IF (cc), IF (p), IHC (fro), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IF 宿主: 小鼠 Monoclonal Luci 21 1-107 unconjugated
anti-Luciferase antibody适用: Firefly ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Luciferase Antibody is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:25,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 75 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).
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: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).
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- 抗原
- Luciferase
- Abstract
- Luciferase 产品
- 别名
- hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody
- 基因ID
- 3280269
- UniProt
- P29238, P19907
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