ACSS2 抗体 (N-Term)
Quick Overview for ACSS2 抗体 (N-Term) (ABIN5706867)
抗原
See all ACSS2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-gw7
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Supplier
- Rockland
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原理
- ACSS2 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human ACSS2 protein.
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产品特性
- Synonyms: rabbit anti-ACSS2, rabbit anti ACSS2, Acetyl-coenzyme A synthetase (cytoplasmic), Acetate-CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACSS2, ACAS2
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纯化方法
- Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-term of human ACSS2.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1 μg/mL for WB. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.
Western Blot Dilution: 1.0 μg/mL
ELISA Dilution: 1:10,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.01 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))
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别名
- ACSS2
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). ACSS2 (Acyl-CoA synthetase short-chain family member 2) is a protein coding gene encoding a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. This protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. This protein is upregulated in many cancers and is important for pro-survival under hypoxic conditions. ACSS2 may be associated with disorders such as Cystoisosporiasis. Anti-ACSS2 Antibody is useful for researchers interested in parasites, intestines, Glucose Energy Metabolism, and AKT research.
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基因ID
- 55902
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NCBI登录号
- NP_001070020
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UniProt
- Q9NR19
抗原
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