ORF2 (C-Term) 抗体
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- 抗原
- ORF2
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抗原表位
- C-Term
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 600-401-gt3
- Supplier
- Rockland
- 原理
- Orf2 Antibody
- 交叉反应 （详细）
- Anti-Orf2 is directed against mouse ORF2 near a c-terminus position.
- 产品特性
- Synonyms: Rabbit Anti-Orf2 Antibody, Endonuclease reverse transcriptase, LINE-1 retrotransposable element ORF2 protein, Line1 protein, Orf2p, retrovirus-related Pol polyprotein LINE-1, Long interspersed element-1, L1, Orf-2
- 纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
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anti-ORF2 antibody
适用: 人, Chimpanzee ELISA, WB, DB 宿主: 小鸡 Polyclonal unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~150 kDa in size corresponding to Orf2 by western blotting in the liver cell lysates or extract.
Western Blot Dilution: 1.0 μg/mL
ELISA Dilution: 1.0 μg/ul
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.07 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- ORF2
- 物质类
- Viral Protein
- 背景
- Background: Anti-Orf2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embyronic development and it is suggested that mutations of L1 would result in detrimental de novo insertions into the genome, negatively affecting gene expression. L1 elements are critical components in the mammalian genome, and much is still not known regarding these proteins. Studies implicate that line-1 is activated in fetal oocytes and L1ORF2p may contribute to the eradication or selection of the oocytes.
- 基因ID
- 2731767
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