Ngp 抗体
This anti-Ngp antibody is a 兔 多克隆 antibody detecting Ngp in FM, IF 和 WB. Suitable for 小鼠. This Primary Antibody has been cited in 1 publication.
(1 reference)
Quick Overview for Ngp 抗体 (ABIN5706813)
抗原
Ngp (NGP)
(Neutrophilic Granule Protein (NGP))
适用
小鼠
宿主
兔
克隆类型
多克隆
应用范围
Fluorescence Microscopy (FM), Immunofluorescence (IF), Western Blotting (WB)
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Supplier Product No.
- 600-401-gw9
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Supplier
- Rockland
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原理
- Ngp Antibody
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交叉反应 （详细）
- Anti-NGP is directed against full-length mouse NGP.
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产品特性
- Synonyms: rabbit anti-NGP antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Affinity purified Anti-NGP antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse NGP protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This affinity purified antibody has been tested for use in IF, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to NGP by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
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: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
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- Ngp (NGP) (Neutrophilic Granule Protein (NGP))
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别名
- Ngp
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.
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基因ID
- 18054
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NCBI登录号
- NP_032720
抗原
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