Histone H4 抗体 (meLys20)
(1 reference)
Quick Overview for Histone H4 抗体 (meLys20) (ABIN5706797)
抗原
See all Histone H4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- meLys20
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Supplier Product No.
- 600-401-j01
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Supplier
- Rockland
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原理
- Histone H4 K20me1 Antibody
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交叉反应 （详细）
- This antibody reacts with human Histone H4.
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产品特性
- Synonyms: rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
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纯化方法
- Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Histone H4 (acLys12) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (3meLys20) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys12) antibody适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys5) antibody适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone H4 (meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (2meLys20) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (3meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys8) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, Saccharomyces cerevisiae, Drosophila melanogaster WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H4 [Monomethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
Other: Dot Blot 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.12 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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: "ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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- Histone H4
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背景
- Background: SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
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基因ID
- 121504
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NCBI登录号
- NP_001029249
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UniProt
- P62805
抗原
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